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Induction of cytochrome(s) P 450 ‐dependent drug metabolism in cultured MH 1 C 1 hepatoma cells
Author(s) -
Ferro Margherita,
Bassi Anna M.,
Marinari Umberto M.,
Nanni Giorgio,
Chiarpotto Elena,
Poli Giuseppe,
Dianzani Mario U.
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020415
Subject(s) - metyrapone , phenobarbital , inducer , cytochrome p450 , pyrene , chemistry , monooxygenase , enzyme inducer , cytochrome , demethylase , drug metabolism , enzyme , methylcholanthrene , microsome , cell culture , metabolism , stimulation , biochemistry , biology , pharmacology , carcinogen , endocrinology , organic chemistry , genetics , gene , epigenetics
A cell line derived from a Morris hepatoma, MH 1 C 1 , was examined for its in vitro expression of monooxygenases. These cells were found to contain different forms of cytochrome P 450 , as shown by the response to inducers, namely phenobarbital (PB), 3‐methylcholanthrene (MC) and metyrapone (MP). MH 1 C 1 cell monolayers exposed to PB or MC showed an increase in the concentration of two spectrally distinct forms of cytochrome P 450 . The PB and MC treatments elicited enzyme activities towards the substrates aminopyrine and benzo( a )pyrene, respectively. The cell treatment with metyrapone led to a simultaneous stimulation of aminopyrine demethylase and benzo(a)pyrene hydroxylase activities, so underlining the peculiar features of this inducer.