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Changes in enzyme activities involved in the degradation of 1,3‐bisphosphoglycerate during erythropoiesis in rat bone marrow
Author(s) -
Pineda Manuel,
Pinilla Montserrat,
Luque José
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020413
Subject(s) - percoll , erythropoiesis , phosphoglycerate mutase , phosphoglycerate kinase , bone marrow , biochemistry , enzyme , differential centrifugation , biology , chemistry , kinase , microbiology and biotechnology , glycolysis , medicine , in vitro , immunology , anemia
Rat bone marrow cells have been fractionated by density gradient in Percoll. Differential counting of erythroid cells, haemoglobin concentration and bisphosphoglycerate mutase and phosphoglycerate kinase activities have been determined in cellular fractions. As shown by means of a statistical approach, an increase in bisphosphoglycerate mutase activity and a slight decrease in phosphoglycerate kinase activity is found in erythroid cells as their haemoglobin content increases. Our results suggest that there is a synthesis of 2,3‐bisphosphoglycerate during the erythropoietic process which parallels the synthesis of haemoglobin.