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DNA‐damaging activity of biotic and xenobiotic aldehydes in chinese hamster ovary cells
Author(s) -
Marinari Umberto M.,
Ferro Margherita,
Bassi Anna Maria,
Sciaba Luigi,
Finollo Renata,
Brambilla Giovanni
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020411
Subject(s) - propionaldehyde , methylglyoxal , dna damage , hexanal , chemistry , chinese hamster ovary cell , dna , acetaldehyde , lipid peroxidation , biochemistry , xenobiotic , microbiology and biotechnology , biology , oxidative stress , chromatography , enzyme , ethanol , receptor
Alkaline elution was employed to study DNA damage in CHO‐Kl cells treated with a series of biotic and xenobiotic aldehydes. DNA cross‐linking was measured in terms of the reduction in the effect of methyl methanesulphonate on the kinetics of DNA elution and was observed in cells treated with formaldehyde, acetaldehyde, methylglyoxal and malonaldehyde. Propionaldehyde, valeraldehyde, hexanal and 4‐hydroxynonenal produced DNA single‐strand breaks, or lesions which were converted to breaks in alkali. Both types of DNA damage occurred in cells exposed to malealdehyde. These findings support the hypothesis of a carcinogenic effect of the aldehydic products (malonaldehyde, methylglyoxal, propionaldehyde, hexanal, 4‐hydroxynonenal) released in biomembranes during lipid peroxidation.