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Immunofluorescent staining method for use with monoclonal antibodies
Author(s) -
Costanzo Catherine,
Raza Azra
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020307
Subject(s) - bromodeoxyuridine , monoclonal antibody , staining , dna , monoclonal , antibody , microbiology and biotechnology , biology , chemistry , immunohistochemistry , immunology , biochemistry , genetics
This note describes an immunofluorescent staining method for cells in the S‐phase which have been allowed to take up bromodeoxyuridine into their DNA in place of thymine. The technique involves the use of fluorescinated monoclonal antibodies against bromodeoxyuridine and allows rapid and accurate estimation of cells in the S‐phase, the technique does not require interpretation by skilled technicians.