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Stimulation of rat renal Na + , K + ‐ATPase activity by thyroid hormones
Author(s) -
Pippard Corinna,
Baylis P. H.
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020210
Subject(s) - stimulation , endocrinology , medicine , renal cortex , kidney , chemistry , antiserum , hormone , thyroid , enzyme assay , atpase , enzyme , renal medulla , biology , biochemistry , antibody , immunology
The effect of thyroid hormones (T4, T3 and reverse T3) on rat renal Na + , K + ‐ATPase activity was investigated by a cytochemical technique. T3 caused stimulation of Na + , K + ‐ATPase activity in the renal medulla but not in the renal cortex. There was a peak in enzyme activity after cultured renal segments had been exposed to T3 for 11 min and this time of maximal stimulation did not vary with the concentration of T3. A rectilinear response in Na + , K + ‐ATPase activity was observed over T3 concentration range 10 pmol l −1 to 100 nmol l −1 ; at higher T3 concentrations, Na + ,K + ‐ATPase activity was inhibited. The enzyme response was totally blocked by specific T3 antiserum. Addition of T4 and reverse T3 (100 fmol l −1 − 1 mmol l −1 ) failed to stimulate Na + ,K + ‐ATPase activity in any part of the kidney. Plasma (neat and diluted 1:10) stimulated the enzyme in parallel with the dose response curve and the stimulatory effect was abolished by prior addition of specific T3 antiserum.