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Integrity of glycoprotein complex sugars is required for homing but not for several other membrane‐mediated functions
Author(s) -
Hooghe Robert J.,
Plaetse Frank Vander,
de SaintGeorges Louis,
Janowski Michel,
SchaafLafontaine Nicole,
HooghePeters Elisabeth L.,
Dorling Peter
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020209
Subject(s) - swainsonine , glycoprotein , biochemistry , in vivo , membrane glycoproteins , homing (biology) , isoelectric point , cell , mannose , biology , sialic acid , chemistry , microbiology and biotechnology , enzyme , genetics , ecology
In order to correlate the biochemistry of cell surface carbohydrates with cell function, we have treated cells with swainsonine and followed the biochemical and functional modifications induced by this compound. After treatment with swainsonine, surface glycoproteins had a lower apparent molecular weight and a higher isoelectric point. This is compatible with the replacement of complex carbohydrates by hybrid or high‐mannose carbohydrates. Several functional tests were unaffected. However, swainsonine‐treated cells displayed an altered pattern of in vivo homing, suggesting that carbohydrates play a role in this process.