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Distribution of non‐histone proteins between micrococcal nuclease sensitive and nuclease resistant chromatin from chicken cells with active and inactive genomes
Author(s) -
Kiliańska Z.,
KłyszejkoStefanowicz L.
Publication year - 1984
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290020205
Subject(s) - micrococcal nuclease , chromatin , nuclease , histone , microbiology and biotechnology , polyacrylamide gel electrophoresis , gel electrophoresis , biology , biochemistry , in vitro , dna , non histone protein , chemistry , enzyme , nucleosome
Chicken liver and erythrocyte nuclei were separated by mild treatment with micrococcal nuclease into nuclease sensitive (NS) and nuclease resistant (NR) fractions, differing in chemical composition and transcriptional activity in vitro. Nuclei, NS and NR fractions of both tissues were fractionated by hydroxyapatite chromatography into three groups of non‐histone chromatin proteins (NHCP) and characterized by SDS‐polyacrylamide gel electrophoresis. Some differences in the molecular distribution of non‐histone proteins of chicken liver and erythrocytes between nuclease sensitive and resistant parts of chromatin have been described.