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Is there any connection between heat inactivation of spectrin‐dependent ATPase and loss of smooth biconcave shape of red cells?
Author(s) -
Mirčevová Libuše,
Kodíček Milan,
Mařík Tomáš
Publication year - 1983
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290010305
Subject(s) - spectrin , atpase , chemistry , biophysics , membrane , microbiology and biotechnology , erythrocyte membrane , biochemistry , biology , enzyme , cytoskeleton , cell
Spectrin‐dependent ATPase activity was measured in membranes from native human erythrocytes and erythrocytes heated for 20 min at different temperatures. This activity was found to decline when the erythrocytes were heated at 48°C and higher. The break in ATPase activity corresponds to morphological changes in erythrocytes found by Crome and Mollioson [Brit. J. Haematol. (1964) 10, 137]. The role of spectrin‐dependent ATPase in erythrocyte shape maintenance is discussed.