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Hepatoprotective effect of bis (4‐methylbenzoyl) diselenide against CCl 4 ‐induced oxidative damage in mice
Author(s) -
Filho Carlos Borges,
Del Fabbro Lucian,
Boeira Silvana P.,
Furian Ana Flávia,
Savegnago Lucielli,
Soares Letiére Cabreira,
Braga Antonio Luiz,
Jesse Cristiano R.
Publication year - 2013
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2869
Subject(s) - alkaline phosphatase , chemistry , carbon tetrachloride , catalase , lactate dehydrogenase , antioxidant , superoxide dismutase , oxidative stress , thiobarbituric acid , glutathione , ascorbic acid , endocrinology , biochemistry , medicine , pharmacology , lipid peroxidation , enzyme , food science , organic chemistry
From a pharmacological point of view, organoseleniums are compounds with important and interesting antioxidant and biological activities. The aim of this study was to evaluate the hepatoprotective effect of bis (4‐methylbenzoyl) diselenide (BMD) against carbon tetrachloride (CCl 4 )–induced oxidative damage in mice. The animals received BMD (25 mg/kg p.o., for 3 days), and after 1 day, CCl 4 (1 mg/kg body weight) was administered by intraperitoneal route. One day after the CCl 4 exposure, the animals were euthanized for biochemical and histological analysis. Treatment with BMD (25 mg/kg p.o.) protected against aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma‐glutamyl transferase and lactate dehydrogenase activity increases induced by CCl 4 plasma exposure. Treatment with BMD (25 mg/kg) protected against increases in thiobarbituric reactive species and decreasing non‐protein thiols and ascorbic acid levels in liver of mice. Catalase and superoxide dismutase activity inhibition in the liver caused by CCl 4 were protected by treatment with BMD (25 mg/kg). Glutathione S‐transferase activity was inhibited by CCl 4 and remained unaltered even after treatment with BMD. Sections of liver from CCl 4 ‐exposed mice presented an intense infiltration of inflammatory cells and loss of the cellular architecture. BMD (25 mg/kg) attenuated CCl 4 ‐induced hepatic histological alterations. The results demonstrated the hepatoprotective effects of BMD in the mouse liver, possibly by modulating the antioxidant status. Copyright © 2012 John Wiley & Sons, Ltd.