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Interleukin 6 protects H 2 O 2 ‐induced cardiomyocytes injury through upregulation of prohibitin via STAT3 phosphorylation
Author(s) -
Jia Yuhua,
Zhou Fenghua,
Deng Peng,
Fan Qin,
Li Chunhua,
Liu Yawei,
Fu Xiuqiong,
Zhou Yingchun,
Xu Xin,
Sun Xuegang
Publication year - 2012
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2820
Subject(s) - prohibitin , downregulation and upregulation , phosphorylation , western blot , stat3 , small interfering rna , chemistry , apoptosis , microbiology and biotechnology , biology , biochemistry , rna , gene
Objective: Hydrogen peroxide (H 2 O 2 ) is a potent reactive oxygen species that causes cardiomyocytes injury. As an important cytokine, interleukin 6 (IL‐6) has cardioprotective effects as it plays an essential role in the late phase of preconditioning. Our work is to investigate if IL‐6 preconditioning has protective effects on neonatal rat ventricular cardiomyocytes in response to H 2 O 2 and its underlying mechanism. Methods: Gel‐based comparative proteomic approach along with small interfering RNA (siRNA) and Western blot analysis was used to analyse mechanisms of IL‐6 preconditioning on H 2 O 2 ‐induced neonatal rat ventricular cardiomyocytes injury. Results: IL‐6 preconditioning protected cardiomyocytes against H 2 O 2 ‐induced cell death. Proteomic analysis showed that IL‐6 pretreatment further increased the expression of prohibitin and improved the viability of cardiomyocytes exposed to H 2 O 2 . Knocking down of prohibitin with siRNA abrogated this protection by increasing apoptosis rate. Tyrosine kinase inhibitor AG490 decreased signal transducers and activators of transcription 3 (STAT3) phosphorylation and down‐regulated prohibitin expression in cardiomyocytes pretreated with IL‐6 and exposed to H 2 O 2 , which further dampened the protective effects of IL‐6 preconditioning. Conclusion: Our results provide direct evidence that prohibitin is a protective factor of IL‐6 preconditioning in H 2 O 2 ‐induced neonatal rat ventricular cardiomyocytes death. The upregulation of prohibitin by IL‐6 is, at least, partially regulated through STAT3 phosphorylation. Copyright © 2012 John Wiley & Sons, Ltd.