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miRNAs expressed differently in cancer stem cells and cancer cells of human gastric cancer cell line MKN‐45
Author(s) -
Golestaneh Azadeh Fahim,
Atashi Amir,
Langroudi Lida,
Shafiee Abbas,
Ghaemi Nasser,
Soleimani Masoud
Publication year - 2012
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2815
Subject(s) - homeobox protein nanog , sox2 , cancer stem cell , cd44 , cancer cell , microrna , cancer , biology , cell culture , cancer research , lin28 , microbiology and biotechnology , stem cell marker , stem cell , cell , gene , genetics , embryonic stem cell , induced pluripotent stem cell
Recent studies show that cancers may originate from special cells named cancer stem cells (CSCs). As miRNAs have a prominent role in regulating cell activities, a question arise, that is, if there is any difference in miRNA expression level between CSC and other cancer cells of human gastric cancer cell line MKN‐45. In this study, CSCs were isolated by fluorescence‐activated cell sorter based on the expression level of cell surface marker CD44. CSC characteristics were checked using spheroid formation assay and soft agar assay. Using reverse transcriptase polymerase chain reaction (RT‐PCR), the expression level of some stemness genes was studied. Real‐time q‐PCR was used for analysis of the expression level of miRNAs. CSCs were able to make spheroids and colonies, whereas other cancer cells failed to show aforementioned features. In addition, RT‐PCR resulted in a difference in the expression levels of Nanog, Sox2, Lin28 and Oct‐4 between these two kinds of cells. Real‐time RT‐PCR analysis demonstrated an increase in mir‐21 and mir‐302 expression level in CSCs, relative to cancer cells, whereas let‐7a expression level was decreased in CSC in comparison with cancer cells, which may be due to their different differentiation level. On the other hand, mir‐372, mir‐373 and mir‐520c‐5p were markedly increased in cancer cells in comparison with CSCs. This study shows that there is a difference in miRNA expression level between CSCs and other cancer cells, which reflects dissimilar molecular pathways in these cells. These miRNAs may be promising objects for targeting CSCs specifically and efficiently. Copyright © 2012 John Wiley & Sons, Ltd.