Premium
The effects of garlic‐derived sulfur compounds on cell proliferation, caspase 3 activity, thiol levels and anaerobic sulfur metabolism in human hepatoblastoma HepG2 cells
Author(s) -
Iciek Małgorzata,
Kwiecień Inga,
Chwatko Grażyna,
SokołowskaJeżewicz Maria,
KowalczykPachel Danuta,
Rokita Hanna
Publication year - 2012
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1835
Subject(s) - thiol , sulfurtransferase , chemistry , diallyl trisulfide , rhodanese , sulfur , glutathione , biochemistry , diallyl disulfide , cysteine , cell growth , caspase , sulfur metabolism , programmed cell death , caspase 3 , enzyme assay , apoptosis , enzyme , organic chemistry
The aim of the present studies was to determine whether the mechanism of biological action of garlic‐derived sulfur compounds in human hepatoma (HepG2) cells can be dependent on the presence of labile sulfane sulfur in their molecules. We investigated the effect of allyl sulfides from garlic: monosulfide, disulfide and trisulfide on cell proliferation and viability, caspase 3 activity and hydrogen peroxide (H 2 O 2 ) production in HepG2 cells. In parallel, we also examined the influence of the previously mentioned compounds on the levels of thiols, glutathione, cysteine and cysteinyl‐glycine, and on the level of sulfane sulfur and the activity of its metabolic enzymes: rhodanese, 3‐mercaptopyruvate sulfurtransferase and cystathionase. Among the compounds under study, diallyl trisulfide (DATS), a sulfane sulfur‐containing compound, showed the highest biological activity in HepG2 cells. This compound increased the H 2 O 2 formation, lowered the thiol level and produced the strongest inhibition of cell proliferation and the greatest induction of caspase 3 activity in HepG2 cells. DATS did not affect the activity of sulfurtransferases and lowered sulfane sulfur level in HepG2 cells. It appears that sulfane sulfur containing DATS can be bioreduced in cancer cells to hydroperthiol that leads to H 2 O 2 generation, thereby influencing transmission of signals regulating cell proliferation and apoptosis. Copyright © 2011 John Wiley & Sons, Ltd.