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NHE1 mediates migration and invasion of HeLa cells via regulating the expression and localization of MT1‐MMP
Author(s) -
Lin Yani,
Wang Jian,
Jin Weina,
Wang Lihong,
Li Huawen,
Ma Li,
Li Qinghua,
Pang Tianxiang
Publication year - 2012
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1815
Subject(s) - hela , matrix metalloproteinase , extracellular matrix , microbiology and biotechnology , intracellular ph , cell migration , intracellular , extracellular , chemistry , metastasis , regulator , messenger rna , cancer cell , cancer research , cell , biology , cancer , biochemistry , gene , genetics
Na + /H + exchanger 1 (NHE1), acting as an important regulator of intracellular pH (pH i ) and extracellular pH (pH e ), has been known to play a key role in the metastasis of many solid tumours. However, the exact mechanism underlying these processes, especially in cervical cancer, is still poorly understood. In the current study, we first showed that the inhibition of NHE1 activity by the specific inhibitor cariporide could suppress migration and invasion of HeLa cells in vitro . Moreover, cariporide also reversed the enhanced migration and invasion in HeLa cells by overexpressed membrane‐type 1 matrix metalloproteinase (MT1‐MMP). Subsequently, our results showed that NHE1 regulated the expression of MT1‐MMP at both messenger RNA and protein levels as well as its localization. Meanwhile, we observed slight modification in the morphology of HeLa cell after treating with cariporide. The present work indicates that NHE1 mediates HeLa cell metastasis via regulating the expression and localization of MT1‐MMP and provides a theoretical basis for the development of novel therapeutic strategies targeting cervical cancer. Copyright © 2011 John Wiley & Sons, Ltd.