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Rat lymphocytes express NMDA receptors that take part in regulation of cytokine production
Author(s) -
Vladychenskaya Elizaveta,
Tyulina Olga,
Urano Shiro,
Boldyrev Alexander
Publication year - 2011
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1771
Subject(s) - nmda receptor , receptor , chemistry , nicotinamide adenine dinucleotide phosphate , nitric oxide , biochemistry , nitric oxide synthase , ionotropic effect , oxidase test , biology , endocrinology , enzyme
Incubation of rat lymphocytes with homocysteine (HC) or homocysteic acid (HCA) was found to increase the stationary levels of free radicals in lymphocytes, the effect of both ligands being mediated by ionotropic receptors activated by N‐methyl‐D‐aspactic acid (NMDA), the expression of which on rat lymphocyte membranes was earlier demonstrated. In agreement with these data, increase of free radicals in the lymphocyte cytoplasm is preceded by an increase in the intracellular calcium levels, activation of protein kinase C, nicotinamide adenine dinucleotide phosphate oxidase and/or nitric oxide synthase. Both HC and HCA increase the production of IFN‐ γ and TNF‐ α by lymphocytes and antagonist of NMDA receptors; MK‐801 prevents this effect. The data presented show that rat lymphocyte membrane contains functionally active NMDA receptors, which regulate cytokine accumulation. Copyright © 2011 John Wiley & Sons, Ltd.