Premium
Effects of erythropoietin on ICAM‐1 and PECAM‐1 expressions on human umbilical vein endothelial cells subjected to oxidative stress
Author(s) -
Kamianowska Monika,
Szczepański Marek,
Skrzydlewska Elżbieta
Publication year - 2011
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1768
Subject(s) - umbilical vein , erythropoietin , cd31 , oxidative stress , tumor necrosis factor alpha , icam 1 , flow cytometry , chemistry , intercellular adhesion molecule 1 , intracellular , endocrinology , medicine , pharmacology , biochemistry , immunology , biology , in vitro
The protective effect of erythropoietin (Epo) is based on its ability to reduce oxidation and to stabilize the cells. The aim of the study was to evaluate the influence of Epo on malonyl dialdehyde (MDA), intercellular adhesion molecule‐1 (ICAM‐1) (CD54) and platelet–endothelial cell adhesion molecule‐1 (PECAM‐1) (CD31) levels on human umbilical vein endothelial cells (HUVECs) stimulated by tumour necrosis factor‐α (TNF‐α). HUVECs were incubated with Epo (10–40 IU ml −1 ) or TNF‐α (10–40 ng ml −1 ) alone or preincubated with Epo (20 IU ml −1 ) and subsequently stimulated with TNF‐α (10–40 ng ml −1 ). MDA concentrations were measured using the high‐performance liquid chromatography, whereas ICAM‐1 and PECAM‐1 expressions were evaluated by flow cytometry. Incubation with Epo resulted in a decrease in MDA and the increased expressions of ICAM‐1 and PECAM‐1. Exposure to TNF‐α reflected an increase in MDA, ICAM‐1 and PECAM‐1 levels. These changes were inhibited by preincubation with Epo. The cytoprotective activity proven in this study points to new applications and therapeutic possibilities for Epo. Copyright © 2011 John Wiley & Sons, Ltd.