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Inhibitory effects of novel integrin‐targeting peptides on angiogenesis activity in HUVEC cells in vitro
Author(s) -
Liu Yaqin,
Li Yesen,
Xu Yuhong
Publication year - 2011
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1767
Subject(s) - integrin , angiogenesis , extracellular matrix , motility , microbiology and biotechnology , cell adhesion , cytoskeleton , rgd motif , umbilical vein , cell growth , actin cytoskeleton , chemistry , in vitro , cell , biology , biochemistry , cancer research
Integrins are critically involved in many tumour‐promoting activities. The development of inhibitors against integrins may suppress tumour growth by inhibiting tumour angiogenic signalling. In this study, we investigated the effects of two novel peptides containing the integrin binding arginine‐glycine‐aspartic acid‐motif on inhibiting diverse cell behaviours, including cell adhesion, motility, invasion, tube formation and cell cytoskeleton. Cell adhesion and motility assays demonstrated that cyclopeptides c‐Gly and c‐Lys might inhibit the adhesive and motile activity at the concentration of 25 μM. There was no significant effect on cell invasion, indicating the importance of extracellular matrix degradation in modulating the anti‐invasive effect of human umbilical vein endothelial cells (HUVECs). More importantly, the tubular network formation of HUVECs was significantly inhibited by cyclopeptide c‐Lys besides causing a remarkable inhibition of cytoskeletal organization, disrupting the focal adhesion and actin stress fibres formation. In conclusion, this study results indicated that the novel peptide c‐Lys has the ability to inhibit diverse cell behaviours of HUVECs, and the effects may be mediated at different levels of the tumour growth. Therefore, c‐Lys is perhaps proposed to be a potent anti‐angiogenic drug candidate. Copyright © 2011 John Wiley & Sons, Ltd.