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Reduced activity and cytoplasmic localization of Runx2 is observed in C3h10t1/2 cells overexpressing Tbx3
Author(s) -
Deepak Vishwa,
Zhang Zhongli,
Meng Lingyi,
Zeng Xianlu,
Liu Wenguang
Publication year - 2011
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1753
Subject(s) - runx2 , cytoplasm , osteopontin , transcription factor , immunofluorescence , microbiology and biotechnology , luciferase , chemistry , nucleus , transcription (linguistics) , biology , transfection , gene , immunology , biochemistry , antibody , linguistics , philosophy
Tbox3, a T‐box containing transcription factor, has been reported to negatively regulate osteoblastogenesis. Here, we studied the effects mediated by Tbx3 on the master osteogenic transcription factor Runx2 in C3h10t1/2 cells. Dual‐luciferase assay results showed that Tbx3 interferes in the Runx2‐mediated activation of osteopontin promoter by abrogating Runx2 activity. Immunofluorescence study demonstrated that Runx2 was simultaneously localized in the nucleus and the cytoplasm of Tbx3‐overexpressing cells. The results obtained in this study indicate that Tbx3 abrogates Runx2 activity and elevated expression levels of Tbx3 in the cells can result in mislocalization of Runx2. Copyright © 2011 John Wiley & Sons, Ltd.