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A novel composition for in vitro and in vivo regeneration of skin and connective tissues
Author(s) -
Gennero Luisa,
De Siena Rocco,
Denysenko Tetyana,
Roos Maria Augusta,
Calisti Gian Franco,
Martano Manuela,
Fiobellot Simona,
Panzone Michele,
Reguzzi Stefano,
Gabetti Luisa,
Vercelli Andrea,
Cavallo Giovanni,
Ricci Elia,
Pescarmona Gian Piero
Publication year - 2011
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1751
Subject(s) - regeneration (biology) , in vivo , wound healing , connective tissue , in vitro , pharmacology , skin repair , pathology , medicine , chemistry , biology , microbiology and biotechnology , surgery , biochemistry
The particular combination of polydeoxyribonucleotides, l ‐carnitine, calcium ions, proteolytic enzyme and other ingredients acts in a synergetic way in the regeneration of skin and connective tissues. This new formulation of active principles was tested in vitro as a cell and tissue culture medium and in vivo for various preparations in support of tissue regeneration. In vitro , the new blend allowed the maintenance of skin biopsies for more than 1 year in eutrophic conditions. Immunocytochemical analyses of fibroblasts isolated from these biopsies confirmed a significant increase of the epidermal and connective wound‐healing markers such as collagen type I, collagen type IV, cytokeratin 1 (CK1), CK5, CK10 and CK14 versus controls. To examine the effects of the new compound in vivo , we studied impaired wound healing in genetically diabetic db/db mice. At day 18, diabetic mice treated with the new composition showed 100% closure of wounds and faster healing than mice treated with the other solutions. This complex of vital continuity factors or life‐keeping factors could be used as a tissue‐preserving solution or a cosmetic/drug/medical device to accelerate wound healing in the treatment of patients with deficient wound repair to promote the regeneration of cutaneous and connective tissues (injuries–wound, dermatitis) and prevent the recurrent relapses. Copyright © 2011 John Wiley & Sons, Ltd.