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Immunomodulation property of hexane fraction of leaves of Cinnamomum tamala Linn. in rats
Author(s) -
Chaurasia Jitendra K,
Mishra Anshulika,
Tripathi Yamini B
Publication year - 2010
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1677
Subject(s) - bone marrow , mitotic index , spleen , dexamethasone , concanavalin a , white blood cell , in vivo , pharmacology , in vitro , endocrinology , medicine , immunology , chemistry , biology , mitosis , biochemistry , microbiology and biotechnology
The leaves of Cinnamomum tamala Linn. (CT) (Lauraceae) clinically used in Ayurveda as antidiabetic and diuretic, but no reports are available towards immunomodulating property. Its hexane fraction (CTH) was orally given to rats for 10 days and delayed type of hypersensitivity (DTH), antibody producton against sheep red blood cells (SRBCs), mitotic index in bone marrow cells and concanavalin A (Con A) mediated proliferation of lymphocytes were assessed. Further on 30 days treatment, change in body weight (BW), spleen weight, thymus weight, bone marrow cellularity and hematological changes were observed. It inhibited significantly the DTH response (IC 50 1475 ± 57.19 mg kg −1 BW), antibody production, suppressed mitotic index in bone marrow cells along with the suppression of lymphocyte proliferation against Con A (IC 50 63.33 ± 1.95 µg mL −1 ). In all experiments, cyclophasphamide and dexamethasone had been used as reference drug for in vivo and in vitro studies, respectively. On 30 days treatment, the CTH (800 mg kg −1 BW and above) significantly suppressed growth rate, increase of spleen and thymus weight and low bone marrow cellularity. In hematological examination, it inhibited total white blood cell and lymphocytes count and increased per cent of polymorphs. Thus, it could be suggested that the fraction possesses immunosuppressive property at doses, higher than 800 mg kg −1 BW in rats. Copyright © 2010 John Wiley & Sons, Ltd.