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Increased expression of glucose transporter 3 in gerbil brains following magnesium sulfate treatment and focal cerebral ischemic injury
Author(s) -
Huang ChihYang,
Liou YiFan,
Chung ShuYing,
Pai PeiYing,
Kan ChungBen,
Kuo ChiaHua,
Tsai ChangHai,
Tsai FuuJen,
Chen JiaLong,
Lin JingYing
Publication year - 2010
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1659
Subject(s) - glut3 , ischemia , gerbil , hippocampus , medicine , glucose transporter , endocrinology , cerebral cortex , middle cerebral artery , cortex (anatomy) , anesthesia , neuroscience , biology , glut1 , insulin
Glucose is the primary energy substrate for neurons. Glucose transporter 3 (Glut3) localizes at the neuronal cellular membrane, which transports glucose from the extracelluar space into neurons. Ischemia results in an increased energy demand that is associated with profound changes in brain energy metabolism. Magnesium sulfate (MgSO 4 ) ameliorates ischemia‐induced neuronal death in the rat and gerbil model. We investigated the effects of MgSO 4 administration on the expression of Glut3 in cortex and hippocampus of gerbils during ischemia. The focal cerebral ischemia was produced by unilateral occlusion of the right common carotid artery and right middle cerebral artery. Following ischemia, Glut3 expression increased significantly versus non‐ischemic (contra‐lateral) cortex and hippocampus. MgSO 4 treatment significantly increased the level of Glut3 expression in the non‐ischemic and ischemic cortex and hippocampus. We found that the MgSO 4 ‐induced increase in Glut3 expression was not reversed by administration of U0126, a MEK kinase inhibitor. These results suggest that other factors may function to modulate the MgSO 4 ‐induced Glut3 response. In all, our data showed that MgSO 4 increases the expression of Glut3 in the cortex and hippocampus of gerbil brains both in non‐ischemia and ischemia status. However, the MEK signaling pathway might not be involved in MgSO 4 ‐induced Glut3 expression following focal ischemia. Copyright © 2010 John Wiley & Sons, Ltd.