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Soybean and sunflower oil‐induced insulin resistance correlates with impaired GLUT4 protein expression and translocation specifically in white adipose tissue
Author(s) -
Poletto Ana Cláudia,
Anhê Gabriel Forato,
Eichler Paula,
Takahashi Hilton Kenji,
Furuya Daniela Tomie,
Okamoto Maristela Mitiko,
Curi Rui,
Machado Ubiratan Fabres
Publication year - 2010
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1628
Subject(s) - glut4 , insulin resistance , white adipose tissue , chromosomal translocation , adipose tissue , white (mutation) , medicine , endocrinology , sunflower oil , insulin , protein expression , biology , biochemistry , gene
Free fatty acids are known for playing a crucial role in the development of insulin resistance. High fat intake is known for impairing insulin sensitivity; however, the effect of vegetable‐oil injections have never been investigated. The present study investigated the effects of daily subcutaneous injections (100 µL) of soybean (SB) and sunflower (SF) oils, during 7 days. Both treated groups developed insulin resistance as assessed by insulin tolerance test. The mechanism underlying the SB‐ and SF‐induced insulin resistance was shown to involve GLUT4. In SB‐ and SF‐treated animals, the GLUT4 protein expression was reduced ∼20% and 10 min after an acute in vivo stimulus with insulin, the plasma membrane GLUT4 content was ∼60% lower in white adipose tissue (WAT). No effects were observed in skeletal muscle. Additionally, both oil treatments increased mainly the content of palmitic acid (∼150%) in WAT, which can contribute to explain the GLUT4 regulations. Altogether, the present study collects evidence that those oil treatments might generate insulin resistance by targeting GLUT4 expression and translocation specifically in WAT. These alterations are likely to be caused due to the specific local increase in saturated fatty acids that occurred as a consequence of oil daily injections. Copyright © 2010 John Wiley & Sons, Ltd.