Premium
p63 and its isoforms as markers of rat oral mucosa epidermal stem cells in vitro
Author(s) -
Tao Qian,
Qiao Bin,
Lv Biao,
Zheng Chaoqun,
Chen Zhifeng,
Huang Hongzhang
Publication year - 2009
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1612
Subject(s) - in vitro , oral mucosa , stem cell , gene isoform , microbiology and biotechnology , biology , biochemistry , anatomy , gene
Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population of ESCs, whether p63 can function as a specific marker for ESCs is unclear. We isolated and cultured oral ESCs and illustrated the expression of p63 and its isoforms in rat oral mucosa tissues and stem cells before and after differentiation. Semi‐reverse transcription PCR detected the TA, ΔN, α and β isoforms when cells were cultured for 2 days, but only TA and γ were detected after 14 days. We also found that p63 is expressed in basal and suprabasal epithelial layers of rat oral mucosa, but it was implied p63 does not mark stem cells specifically, while the ΔNp63 α and ΔNp63 β isoforms may be specific markers of rat oral mucosa stem cells. Copyright © 2009 John Wiley & Sons, Ltd.