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Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET‐1, TNF‐ α , and IL‐1 α in LPS‐induced rat intestinal microvascular endothelial cells
Author(s) -
Hu Yiyi,
Chen Xi,
Duan Huiqin,
Hu Yuanliang,
Mu Xiang
Publication year - 2009
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1570
Subject(s) - berberine , jatrorrhizine , palmatine , tumor necrosis factor alpha , decoction , pharmacology , lipopolysaccharide , nitric oxide , chemistry , secretion , berberis , medicine , traditional medicine , biochemistry , endocrinology , organic chemistry
To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐ α (TNF‐ α ), and interleukin‐1 α (IL‐1 α ) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD and its seven active ingredients, namely anemoside B 4 , anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml −1 for 3 h and then treated with PD at 1, 5, and 10 mg ml −1 and its seven ingredients at 1, 5, and 10 µg ml −1 for 21 h, respectively. The results revealed that PD, anemonin, berberine, and esculetin inhibited the production of NO; PD, anemonin, and esculetin inhibited the secretion of ET‐1; PD, anemoside B 4 , berberine, jatrorrhizine, and aesculin downregulated TNF‐ α expression; PD, anemoside B 4 , berberine, and palmatine decreased the content of IL‐1 α . It showed that PD and its active ingredients could significantly inhibit the secretion of NO, ET‐1, TNF‐ α , and IL‐1 α in LPS‐induced RIMECs and suggested they would reduce inflammatory response via these cytokines. Copyright © 2009 John Wiley & Sons, Ltd.