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In vitro cardiomyogenic differentiation of adipose‐derived stromal cells using transforming growth factor‐ β 1
Author(s) -
Gwak SoJung,
Bhang Suk H.,
Yang Hee S.,
Kim SangSoo,
Lee DaeHee,
Lee SooHong,
Kim ByungSoo
Publication year - 2009
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1547
Subject(s) - mesenchymal stem cell , stromal cell , microbiology and biotechnology , adipogenesis , transforming growth factor , cellular differentiation , in vitro , stem cell , adipose tissue , biology , immunology , endocrinology , cancer research , gene , biochemistry
Transplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor‐ β 1 (TGF‐ β 1) induces cardiomyogenic differentiation of adipose‐ derived stromal cells (ADSCs) in vitro . Rat ADSCs were cultured with TGF‐ β 1 (10 ng ml −1 ) for 2 weeks in vitro . ADSCs cultured without TGF‐ β 1 served as a control. The mRNA expression of cardiac‐specific gene was induced by TGF‐ β 1, while the control culture did not show cardiac‐specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF‐ β 1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and α ‐sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF‐ β 1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF‐ β 1 induce ADSC cardiomyogenic differentiation in vitro , which could be useful for myocardial infarction stem cell therapy. Copyright © 2009 John Wiley & Sons, Ltd.