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Protective action of indole‐3‐acetic acid on induced hepatocarcinoma in mice
Author(s) -
Mourão Luciana R. M. B.,
Santana Roberta S. S.,
Paulo Lívia M.,
Pugine Silvana M. P.,
Chaible Lucas M.,
Fukumasu Heidge,
Dagli Maria L. Z.,
de Melo Mariza P.
Publication year - 2009
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1528
Subject(s) - glutathione peroxidase , superoxide dismutase , glutathione reductase , chemistry , catalase , antioxidant , glutathione , enzyme , pharmacology , biochemistry , microbiology and biotechnology , biology
Abstract In this study, we report the protective effects of IAA on diethylnitrosamine (DEN)‐induced hepatocarcinogenesis. BALB/c mice received daily IAA at 50 ( T 50 ), 250 ( T 250 ), and 500 ( T 500 ) mg Kg −1 per body mass by gavage for 15 days. At day 15, animals were administered DEN and sacrificed 4 h later. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed in sera. In addition, hepatomorphologic alterations, activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), gene expression of antioxidant enzymes and DNA integrity were evaluated in the liver. IAA administration did not show any alterations in any of the parameters available, except for a reduction of the gene expression for antioxidant enzymes by 55, 56, 27, and 28% for SOD, CAT, GPx, and GR upon T 500 , respectively compared with the control. Several hepatic alterations were observed by DEN exposure. Moreover, IAA administration at 3 doses was shown to provide a total prevention of the active reduction of CAT and GR induced by DEN exposure compared with the control. IAA at T 500 was shown to give partial protection (87, 71, 57, and 90% for respectively SOD, CAT, GPx, and GR) on the down‐regulation of the enzymes induced by DEN and this auxin showed a partial protection (50%) on DEN‐induced DNA fragmentation for both parameters when compared to DEN alone. This work showed IAA hepatocarcinogenesis protection for the first time by means of a DEN‐protective effect on CAT and GR activity, and by affecting antioxidant gene expression and DNA fragmentation. Copyright © 2008 John Wiley & Sons, Ltd.