L ‐Carnitine suppresses oleic acid‐induced membrane permeability transition of mitochondria | Zendy

Oyanagi Eri | Zendy; Yano Hiromi | Zendy; Kato Yasuko | Zendy; Fujita Hirofumi | Zendy; Utsumi Kozo | Zendy; Sasaki Junzo | Zendy

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L ‐Carnitine suppresses oleic acid‐induced membrane permeability transition of mitochondria

Author(s) -

Oyanagi Eri,

Yano Hiromi,

Kato Yasuko,

Fujita Hirofumi,

Utsumi Kozo,

Sasaki Junzo

Publication year - 2008

Publication title -

cell biochemistry and function

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.933

H-Index - 61

eISSN - 1099-0844

pISSN - 0263-6484

DOI - 10.1002/cbf.1506

Subject(s) - carnitine , oleic acid , mitochondrion , chemistry , mitochondrial permeability transition pore , permeability (electromagnetism) , membrane , transition (genetics) , inner mitochondrial membrane , biophysics , membrane permeability , microbiology and biotechnology , biochemistry , biology , apoptosis , programmed cell death , gene

Membrane permeability transition (MPT) of mitochondria has an important role in apoptosis of various cells. The classic type of MPT is characterized by increased Ca 2+ transport, membrane depolarization, swelling, and sensitivity to cyclosporin A. In this study, we investigated whether L ‐carnitine suppresses oleic acid‐induced MPT using isolated mitochondria from rat liver. Oleic acid‐induced MPT in isolated mitochondria, inhibited endogenous respiration, caused membrane depolarization, and increased large amplitude swelling, and cytochrome c (Cyt. c ) release from mitochondria. L ‐Carnitine was indispensable to β ‐oxidation of oleic acid in the mitochondria, and this reaction required ATP and coenzyme A (CoA). In the presence of ATP and CoA, L ‐carnitine stimulated oleic acid oxidation and suppressed the oleic acid‐induced depolarization, swelling, and Cyt. c release. L ‐Carnitine also contributed to maintaining mitochondrial function, which was decreased by the generation of free fatty acids with the passage of time after isolation. These results suggest that L ‐carnitine acts to maintain mitochondrial function and suppresses oleic acid‐mediated MPT through acceleration of β ‐oxidation. Copyright © 2008 John Wiley & Sons, Ltd.

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