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Mitochondrial AIF protein involved in skeletal muscle regeneration
Author(s) -
Qiu Xiaozhong,
Yu Lei,
Lai Guihua,
Wang Leyu,
Chen Bing,
Ouyang Jun
Publication year - 2008
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1483
Subject(s) - apoptosis inducing factor , skeletal muscle , myocyte , cytoplasm , mitochondrion , microbiology and biotechnology , regeneration (biology) , c2c12 , apoptosis , chemistry , biology , biochemistry , anatomy , programmed cell death , myogenesis , caspase
The mitochondrial flavoprotein apoptosis‐inducing factor (AIF) has proved to be either the main mediator of apoptosis or an anti‐apoptotic factor via its putative oxidoreductase and peroxide scavenging activities. We report here that 100 µM hydrogen peroxide (H 2 O 2 ) induced the proliferation of C2C12 myoblasts and over‐expression of AIF simultaneously in vitro . Immunofluorescence showed that the over‐expression of AIF was located in the cytoplasm. The immunopositive AIF was detected in nuclei 27 days after denervation of skeletal muscle, but in the cytoplasm it was detected 27 days after fiber‐damaged skeletal muscle. AIF may be a factor involved in skeletal muscle regeneration. Copyright © 2008 John Wiley & Sons, Ltd.