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Phospholipid and triacylglycerol fatty acid content and pattern in the cardiac endothelium of rats depleted in long‐chain polyunsaturated ω 3 fatty acids
Author(s) -
Carpentier Yvon A.,
Portois Laurence,
Louchami Karim,
Zhang Ying,
Sener Abdullah,
Malaisse Willy J.
Publication year - 2008
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1392
Subject(s) - polyunsaturated fatty acid , phospholipid , fatty acid , endocrinology , medicine , chemistry , food science , biochemistry , biology , membrane
Rats depleted in long‐chain polyunsaturated ω 3 fatty acids ( ω 3‐depleted rats) display several features of the metabolic syndrome including hypertension and cardiac hypertrophy. This coincides with alteration of the cardiac muscle phospholipid and triacylglycerol fatty acid content and/or pattern. In the present study, the latter variables were measured in the cardiac endothelium of normal and ω 3‐depleted rats. Samples derived from four rats each were obtained from 16 female normal fed rats and three groups of 36–40 female fed ω 3‐depleted rats each aged 8–9, 15–16 and 22–23 weeks. At comparable mean age, the ratio between the square root of the total fatty acid content of phospholipids and cubic root of the total fatty acid content of triacylglycerols was lower in ω 3‐depleted rats than in control animals. The total fatty acid content of triacylglycerols was inversely related to their relative content in C20:4 ω 6. Other differences between ω 3‐depleted rats and control animals consisted in a lower content of long‐chain polyunsaturated ω 3 fatty acids in both phospholipids and triacylglycerols, higher content of long‐chain polyunsaturated ω 6 fatty acids in phospholipids, higher activity of Δ9‐desaturase (C16:0/C16:1 ω 7 and C18:0/C18:1 ω 9 ratios) and elongase [(C16:0 + C16:1 ω 7)/(C18:0 + C18:1 ω 9) and C20:4 ω 6/C22:4 ω 6 ratios], but impaired generation of C22:6 ω 3 from C22:5 ω 3 in the former rats. These findings support the view that cardiovascular perturbations previously documented in the ω 3‐depleted rats may involve impaired heart endothelial function. Copyright © 2007 John Wiley & Sons, Ltd.