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Staurosporine induces tyrosine phosphorylation in Dictyostelium discoideum proteins
Author(s) -
Andrioli Luiz Paulo,
Souza Glaucia Mendes,
da Silva Aline Maria
Publication year - 2006
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1368
Subject(s) - dictyostelium discoideum , staurosporine , phosphorylation , microbiology and biotechnology , tyrosine phosphorylation , dictyostelium , tyrosine kinase , biology , kinase , tyrosine , cell , biochemistry , protein kinase a , signal transduction , gene
The treatment of cells with staurosporine results in inhibition and less frequently activation of protein kinases, in a cell‐type specific manner. In the social amoeba Dictyostelium discoideum , staurosporine induces marked changes in cell morphology affecting growth and development. Here we describe that incubation of D. discoideum growing or starved cells with staurosporine results in a rapid and unexpected tyrosine phosphorylation on two polypeptides of ∼64 and ∼62 kDa. These proteins emerge as novel substrates for tyrosine phosphorylation opening up new perspectives for the study of cell signalling in D. discoideum. Copyright © 2006 John Wiley & Sons, Ltd.