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Protective effect of α‐tocopherol on oxidative stress in experimental pulmonary fibrosis in rats
Author(s) -
Deger Yeter,
Yur Fatmagul,
Ertekin Ali,
Mert Nihat,
Dede Semiha,
Mert Handan
Publication year - 2006
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1362
Subject(s) - bleomycin , malondialdehyde , oxidative stress , pulmonary fibrosis , glutathione , saline , medicine , endocrinology , vitamin e , catalase , antioxidant , lung , chemistry , vitamin , pharmacology , biochemistry , chemotherapy , enzyme
The study was undertaken to investigate the influence of α‐tocopherol (vitamin E) on malondialdehyde (MDA) and glutathione (GSH) levels and catalase (CAT) activity in lung of rats with bleomycin‐induced pulmonary fibrosis (PF). Fourteen Wistar–albino rats were randomly divided into two groups of seven animals each. The first group was treated intra‐tracheally with bleomycin hydrochloride (BM group); the second group was also instilled with BM but received injections of α‐tocopherol twice a week (BM + E group). The third group was treated in the same manner with saline solution only, acting as controls (C). There were decreases in GSH level and CAT activity while an increase in MDA level in BM group was found compared to the control group ( p < 0.05). Vitamin E had a regulator effect on these parameters. After administration of α‐tocopherol, the increase in GSH level and CAT activity and the decrease in MDA level were seen in BM + E group compared to BM group ( p < 0.05). Distinct histopathological changes were found in the BM group compared to the untreated rats. Less severe fibrotic lesions were also observed in the BM + E group. The results show that vitamin E is effective on the prevention of BM‐induced PF, as indicated by differences in the lung levels of oxidants and antioxidants. Copyright © 2006 John Wiley & Sons, Ltd.