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Lipid peroxidation in ovariectomized and pinealectomized rats: the effects of estradiol and progesterone supplementation
Author(s) -
Oztekin Esma,
Baltaci Abdulkerim Kasim,
Tiftik Ali Muhtar,
Mogulkoc Rasim
Publication year - 2006
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1360
Subject(s) - ovariectomized rat , pinealectomy , medicine , endocrinology , lipid peroxidation , glutathione , malondialdehyde , chemistry , antioxidant , glutathione peroxidase , hormone , oxidative stress , melatonin , pineal gland , biochemistry , superoxide dismutase , enzyme
In the present study, we investigated the effect of estradiol and progesterone supplementation on oxidant and antioxidant parameters of renal tissue in ovariectomized and pinealectomized rats. The study was carried out on 36 adult, Spraque–Dawley strain female rats, 6 months of age and weighing 200–250 g. The rats were divided into six groups, each group included six rats: Group 1: Sham‐ovariectomized (Sham‐Ovx) Group 2: Ovariectomized (Ovx) Group 3: Ovx and estradiol (E) and progesterone (P) supplemented (Ovx+E‐P) Group 4: Ovariectomized and sham pinealectomy (Ovx+sham Pnx) Group 5: Ovariectomized+Pinealectomized (Ovx+Pnx) Group 6: Ovariectomized+Pinealectomized+Hormone Supplemented group (Ovx+Pnx+E‐P)The levels of malondialdehyde (MDA), reduced glutathione (GSH) and glutathione peroxidase (GSH‐Px) were analysed in renal tissues of rats. The highest and the lowest levels of MDA were determined in Groups 5 and 1 respectively ( p < 0.001). However, GSH and GSH‐Px levels demonstrated statistically important decreases in groups 2, 4, 5 ( p < 0.001). The findings of this study demonstrate that ovariectomy leads to oxidative damage in renal tissue. Pinealectomy in addition to ovariectomy greatly increases the oxidative damage. However, female sex hormones supplementations to the Ovx and/or Ovx+Pnx rats protected against lipid peroxidation by activating the antioxidant system. Copyright © 2006 John Wiley & Sons, Ltd.