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Effects of PKC ζ on early genome transcription activation in mouse 1‐cell stage fertilized eggs
Author(s) -
Yu Bingzhi,
Fu Wei,
Su Wenhui,
Yu Dahai,
Zhang Zhe,
Feng Chen
Publication year - 2006
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1357
Subject(s) - calphostin c , protein kinase c , transcription (linguistics) , calphostin , biology , microbiology and biotechnology , cell , transcription factor , embryogenesis , embryo , kinase , genetics , gene , linguistics , philosophy
Effects of PKC ζ on the activation of embryonic transcription in 1‐cell stage fertilized mouse eggs were explored. The effects of PKC antagonist calphostin C and PKC ζ specific inhibitor on the activation of embryonic early transcription were observed by Western blotting and cell immunofluorescence. PKC activity increased gradually from G 1 phase to late G 2 phase in mouse 1‐cell stage fertilized eggs, and reached a maximum in G 2 stage. Calphostin C inhibited PKC activity by about 47% in 1‐cell stage fertilized eggs. Calphostin C inhibited early transcription in 1‐cell stage fertilized eggs ( p < 0.01). PKC ζ‐Thr410 in G 2 were about 27% and 110% higher than those in G 1 phase of 1‐cell stage fertilized eggs and MII oocytes, respectively. PKC ζ specific inhibitor can also inhibit early transcription in 1‐cell stage fertilized eggs ( p < 0.05). The results suggest that PKC ζ participates in early transcription activation in mouse 1‐cell stage fertilized eggs. Copyright © 2006 John Wiley & Sons, Ltd.