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Lipid peroxidation in liver tissue of ovariectomized and pinealectomized rats: effect of estradiol and progesterone supplementation
Author(s) -
Oztekin Esma,
Tiftik Ali Muhtar,
Baltaci Abdulkerim Kasim,
Mogulkoc Rasim
Publication year - 2006
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1313
Subject(s) - ovariectomized rat , pinealectomy , medicine , endocrinology , lipid peroxidation , malondialdehyde , glutathione , chemistry , glutathione peroxidase , hormone , melatonin , oxidative stress , biochemistry , pineal gland , superoxide dismutase , enzyme
The present study aimed to determine the effect of estradiol‐progesterone supplementation and pinealectomy on lipid peroxidation of liver tissue in ovariectomized rats. The study was carried out on 36 adult Sprague‐Dawley female rats, which weighed 200–250 g. The rats were divided into 6 groups: Group 1: Sham Ovariectomy (Sham‐Ovx), Group 2: Ovariectomy (Ovx), Group 3: Ovx + Estradiol‐Progesterone supplementation (Ovx + H), Group 4: Sham Pinealectomy and Ovx (Sham Pnx ‐Ovx), Group 5: Ovx ‐Pnx, Group 6: Ovx ‐Pnx + H. Malondialdehyde (MDA), reduced form of glutathione (GSH) and glutathione peroxidase (GSH‐Px) levels were determined in liver tissue of rats. The highest MDA levels and the lowest GSH‐Px levels were determined in the ovariectomized‐pinealectomized group, whereas the lowest MDA was in the Sham‐Ovx group, and the highest GSH‐Px levels were found in the Sham‐Ovx and Ovx + Hormone supplemented group. Furthermore, the highest GSH levels were in group 1 and lowest levels were in group 5. The findings of this study demonstrate that ovariectomy led to lipid peroxidation in liver tissues of rats. Pinealectomy in addition to ovariectomy, increases lipid peroxidation, but, estradiol and progesterone supplementations to the ovariectomized‐pinealectomized rats protect against lipid peroxidation to a significant extent. Copyright © 2006 John Wiley & Sons, Ltd.