Premium
Effects of cyclic AMP‐elevating hormones and autacoids on LPS‐activated rat peritoneal, bronchoalveolar and hepatic (Kupffer) macrophages
Author(s) -
Kondomerkos Dimitrios J.,
Kalamidas Stefanos A.,
Kotoulas Othon B.
Publication year - 2006
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1205
Subject(s) - autacoid , hormone , kupffer cell , endocrinology , medicine , chemistry , receptor
Peritoneal, bronchoalveolar and hepatic (Kupffer) macrophages activated in vitro by endotoxin, exhibit alterations in nitric oxide production when certain hormones or other biologically active agents (autacoids) are present in the culture medium. They also show changes in acid β‐glucuronidase activities and morphological changes concerning cell size and general appearance. Agents known to elevate the intracellular levels of cyclic AMP, e.g. adrenalin, prostaglandin E2 and dopamine, increase the nitric oxide production in all three types of macrophage. The addition of H‐89, an inhibitor of protein kinase A, abolishes the increase in nitric oxide production. Adrenalin also increases the extracellular activity of β‐glucuronidase. The results of this work suggest that cyclic AMP‐elevating hormones and autacoids affect the functions of endotoxin‐activated macrophages, such as the production of nitric oxide and the activity of acid β‐glucuronidase. Copyright © 2005 John Wiley & Sons, Ltd.