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Comparative studies of the endogenous phospholipids and their in vitro hydrolysis by endogenous phospholipases of various tissues from 7‐day‐old chicks: a thin layer chromatographic and densitometric analysis
Author(s) -
Helmy Fatma M.
Publication year - 2004
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1170
Subject(s) - endogeny , chromatography , in vitro , phospholipase , chemistry , thin layer , thin layer chromatography , hydrolysis , biochemistry , layer (electronics) , enzyme , organic chemistry
The phospholipid profiles of heart, kidney, and pectoral muscle of 7‐day‐old chicks and their in vitro response to the endogenous lipolytic enzymes (mainly in the phospholipase group) at pH 7.4 and 38°C for 60 min were analysed by TLC technology and densitometry. The noticeable preferential deacylation of cardiolipin (CL) as detected by the formation of monolysocardiolipin (MLCL) and concurrent reduction of CL level were the most prevalent lipolytic events of chick cardiac muscle, but the least prevalent in chick pectoral muscle. Deacylation of ethanolamine plasmalogen (PE) as revealed by the formation of the corresponding lyso alkenyl derivative was also prominent in cardiac muscle, but much less so in kidney and none at all was detected in pectoral muscle. The level of sphingomyelin (SM) was much higher in kidney than heart and pectoral muscle. Following in vitro incubation, the reduction in the level of SM and the high level of ceramide (Cer) production were most conspicuous in kidney, less in cardiac muscle and least in pectoral muscle. The hydrolysis of PE and SM confirm the action of endogenous PLA 2 and endogenous sphingomyelinase on PE and SM respectively. These data clearly illustrate the differential response of the endogenous substrates (phospholipids) to the endogenous phospholipases of the tissues studied and are probably related to their physiological activities in vivo . Copyright © 2004 John Wiley & Sons, Ltd.