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Differential expression of protein kinase C α and δ in testes of mouse at various stages of development
Author(s) -
Feng Chen,
Zhang Jie,
Gasana Vénuste,
Fu Wei,
Liu Yi,
Zong ZhiHong,
Yu BingZhi
Publication year - 2005
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1167
Subject(s) - protein kinase c , spermatid , gene isoform , isozyme , biology , kinase , messenger rna , blot , protein kinase a , threonine , microbiology and biotechnology , serine , phosphorylation , spermatogenesis , biochemistry , enzyme , gene , endocrinology
Protein kinase C (PKC) describes a family of serine/threonine protein kinases, and multiple isoforms are expressed in various mammalian tissues. In the present study, we examined the expression of PKC‐α and PKC‐δ at protein and mRNA level in mouse testis by Western blotting and RT‐PCR. We also examined the expression of both PKC isoenzymes in the developing mouse testis. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC‐α were uniformly distributed; but PKC‐δ expression occurred in the testes of 3‐week‐oldmice, perhaps even at a relatively late stage in spermatid development. The results suggest that each isoenzyme may have different functional roles in processing and modulating physiological cellular responses of spermatogenesis. Copyright © 2004 John Wiley & Sons, Ltd.