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Comparative acute effects of leptin and insulin on gluconeogenesis and ketogenesis in perfused rat liver
Author(s) -
BorbaMurad Glaucia Regina,
Mario Erica Guilhen,
Bassoli Bruna Kempfer,
Bazotte Roberto Barbosa,
de Souza Helenir Medri
Publication year - 2005
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1163
Subject(s) - gluconeogenesis , medicine , ketogenesis , endocrinology , insulin , leptin , chemistry , catabolism , metabolism , glycerol , ketone bodies , biology , biochemistry , obesity
The acute effects of physiological levels of leptin (10 ng ml −1 ) and insulin (20 μU ml −1 ) on hepatic gluconeogenesis and ketogenesis were compared. Leptin or insulin alone decreased ( p < 0.05) the activation of hepatic glucose, L ‐lactate and urea production from L ‐alanine. However, the hepatic glucose production was not modified if leptin was combined with insulin. These results indicated that both, i.e. leptin and insulin, could promote a non‐additive reduction in the rate of catabolism of L ‐alanine. However, in contrast with insulin ( p < 0.05), leptin did not inhibit the activation of hepatic glucose production from pyruvate or glycerol. On the other hand, activation of hepatic production of acetoacetate and β‐hydroxybutyrate from octanoate was not affected by leptin or insulin. Thus, our data demonstrate that the acute effect of leptin on hepatic metabolism was partially similar to insulin (activation of glucose production from L ‐alanine and activation of acetoacetate or β‐hydroxybutyrate production from octanoate) and partially different from insulin (activation of glucose production from pyruvate or glycerol). Copyright © 2004 John Wiley & Sons, Ltd.