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TNF‐α and leptin in experimental liver fibrosis models induced by carbon tetrachloride and by common bile duct ligation
Author(s) -
Bahçecıoğlu İbrahim Halil,
Yalniz Mehmet,
Ataseven Hüseyin,
Bülbüller Nurullah,
Keçecı Muzaffer,
Demırdağ Kutbeddin,
Özercan İbrahim,
Üstündağ Bilal
Publication year - 2004
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1114
Subject(s) - medicine , carbon tetrachloride , leptin , fibrosis , steatosis , endocrinology , inflammation , ligation , ccl4 , gastroenterology , chemistry , obesity , organic chemistry
In this study we investigated TNF‐α and leptin levels in two different liver fibrosis models induced by carbon tetrachloride (CCl 4 ) and common bile duct ligation (CBDL). A total of 36 male rats of Albino‐Wistar strain were allocated to three groups. One of the groups was the control. The second group received 0.15 ml 100 g −1 CCl 4 subcutaneously for 6 weeks, 3 days per week. The third group underwent common bile duct ligation (CBDL) and was monitored for 4 weeks. Histopathological investigation included fibrosis, steatosis and inflammation. Serum IL‐6 and TNF‐α levels were analysed by ELISA methods and leptin was analysed by RIA. Fibrosis and steatosis increased significantly in the CCl 4 group in comparison with the CBDL group ( p < 0.01; p < 0.001). Leptin and TNF‐α levels in CCl 4 group were higher than those in the CBDL and control groups ( p < 0.05). TNF‐α and leptin levels were not related to each another in either the CCl 4 group or the CBDL group ( r =0.22, p > 0.05; r =0.19, p > 0.05). The IL‐6 level was higher in the CCl 4 group in relation to severity of inflammation ( p < 0.05). TNF‐α and leptin levels were higher in animals with liver fibrosis induced by CCl 4 , than they were in those whose liver fibrosis was induced by common bile duct ligation. Leptin and TNF‐α may be less effective on the development of liver fibrosis in the group which underwent common bile duct ligation. Copyright © 2004 John Wiley & Sons, Ltd.