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The effects of the caffeic acid phenethyl ester (CAPE) on erythrocyte membrane damage after hind limb ischaemia–reperfusion
Author(s) -
Tamer Lülüfer,
Sucu Nehir,
Ercan Bahadır,
Ünlü Ali,
Çalıkoğlu Mukadder,
Bilgin Ramazan,
Değirmenci Ulaş,
Atik Uğur
Publication year - 2004
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1102
Subject(s) - caffeic acid phenethyl ester , hindlimb , oxidative stress , cape , reperfusion injury , caffeic acid , ischemia , antioxidant , propolis , chemistry , reactive oxygen species , medicine , pharmacology , anesthesia , biochemistry , traditional medicine , archaeology , history
Reactive oxygen species have been implicated in pathogenesis injury after ischaemia–reperfusion (I/R). Caffeic Acid Phenethyl Ester (CAPE), an active component of honeybee propolis extract, exhibits antioxidant and anti‐inflammatory properties. The aim of this study was to investigate the effects of CAPE on erythrocyte membrane damage after hind limb I/R. Rats were divided into two groups: I/R and I/R with CAPE pre‐treatment. They were anaesthetized with intramuscular ketamine 100 mg kg −1 . A 4‐h I/R period was performed on the right hind limb of all animals. In the CAPE‐treated group, animals received CAPE 10 μ m by intraperitoneal injection 1 h before the reperfusion. At the end of the reperfusion period, a midsternotomy was performed. A 5‐ml blood sample was withdrawn from the ascending aorta for biochemical assays. Serum and erythrocyte membrane MDA levels were significantly lower in the CAPE‐treated group when compared to the I/R group ( p = 0.001 and p <0.001, respectively). Erythrocyte membrane Na + ‐K + ATPases activity in the CAPE‐treated group was significantly higher than the I/R group ( p <0.001). In conclusion, CAPE seems to be effective in protecting against oxidative stress. Therefore, we suggest that in order to decrease I/R injury, pre‐administration of CAPE may be a promising agent for a variety of conditions associated with I/R. Copyright © 2004 John Wiley & Sons, Ltd.