A cytochemical assay for osteoclast cathepsin K activity | Zendy

Dodds Robert A. | Zendy

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A cytochemical assay for osteoclast cathepsin K activity

Author(s) -

Dodds Robert A.

Publication year - 2003

Publication title -

cell biochemistry and function

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.933

H-Index - 61

eISSN - 1099-0844

pISSN - 0263-6484

DOI - 10.1002/cbf.1078

Subject(s) - cathepsin k , osteoclast , cathepsin , bone resorption , proteases , chemistry , biochemistry , cysteine proteinase inhibitors , enzyme , microbiology and biotechnology , biology , in vitro , endocrinology , programmed cell death , apoptosis , caspase

Cathepsin K is a member of the papain superfamily of cysteine proteases and plays a pivotal role in osteoclast‐mediated bone resorption. This enzyme is an excellent target for antiresorptive therapies for osteopenic disorders such as osteoporosis. 1 Although isolated inhibitor studies on purified enzymes is required to discover potent and selective inhibitors of cathepsin K, a quantitative cytochemical assay 2 for cathepsin K would allow inhibitors to be tested on actual osteoclasts within sections of bone. Furthermore cathepsin K activity could be used to identify and analyse osteoclasts at definitive stages of their lifespan. A cytochemical assay is described that localizes osteoclast cathepsin K activity in unfixed, undecalcified cryostat sections of animal and human bone. Copyright © 2003 John Wiley & Sons, Ltd.

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