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Protective effect of glycine supplementation on the levels of lipid peroxidation and antioxidant enzymes in the erythrocyte of rats with alcohol‐induced liver injury
Author(s) -
Senthilkumar R.,
Sengottuvelan M.,
Nalini N.
Publication year - 2003
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1062
Subject(s) - tbars , glutathione peroxidase , lipid peroxidation , chemistry , glutathione , glutathione reductase , superoxide dismutase , antioxidant , oxidative stress , endocrinology , liver injury , medicine , thiobarbituric acid , biochemistry , glycine , pharmacology , enzyme , amino acid
We studied the effect of glycine supplementation on lipid peroxidation and antioxidants in the erythrocyte membrane, plasma and hepatocytes of rats with alcohol‐induced hepatotoxicity. Administering ethanol (20%) for 60 days to male Wistar rats resulted in significantly elevated levels of erythrocyte membrane, plasma and hepatocyte thiobarbituric acid reactive substances (TBARS) as compared with those of the experimental control rats. Decreased activities of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx) and glutathione reductase (GR) were also observed on alcohol supplementation as compared with those of the experimental control rats. Glycine was administered at a dose of 0.6 g kg −1 body weight to rats with alcohol‐induced liver injury, which significantly decreased the levels of TBARS and significantly elevated the activities of SOD, CAT, GSH, GPx and GR in the erythrocyte membrane, plasma and hepatocytes as compared to that of untreated alcohol supplemented rats. Thus, our data indicate that supplementation with glycine offers protection against free radical‐mediated oxidative stress in the erythrocyte membrane, plasma and hepatocytes of animals with alcohol‐induced liver injury. Copyright © 2003 John Wiley & Sons, Ltd.