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The effect of desferrioxamine on peroxynitrite‐induced oxidative damage in erythrocytes
Author(s) -
Ertabak Aytu,
Kutluay Türker,
Ünlü Ali,
Türközkan Nurten,
Çimen Behzat,
Yaman Halil
Publication year - 2004
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1056
Subject(s) - peroxynitrite , chemistry , nitrotyrosine , glutathione , antioxidant , biochemistry , peroxidase , glutathione peroxidase , enzyme , in vitro , gpx4 , oxidative phosphorylation , superoxide , nitric oxide synthase
The aim of this study was to investigate the effect of desferrioxamine on peroxynitrite‐mediated damage in erythrocytes by measuring the 3‐nitrotyrosine level and glutathione peroxidase and Na + ‐K + ATPase activities in vitro . 3‐Nitrotyrosine levels were determined by HPLC; glutathione peroxidase and Na + ‐K + ATPase activities were measured by spectrophotometry. Peroxynitrite increased the 3‐nitrotyrosine level but decreased both enzyme activities. In the presence of desferrioxamine, glutathione peroxidase activity was increased with a decrease in the 3‐nitrotyrosine level. Desferrioxamine was found to possess an important antioxidant activity as assessed in an in vitro system, reducing protein nitration, restorating enzyme activities and maintaining erythrocyte membrane integrity. Copyright © 2004 John Wiley & Sons, Ltd.