Premium
NBD‐cholesterol incorporation by rat macrophages and lymphocytes: a process dependent on the activation state of the cells
Author(s) -
Portioli Silva Érica P.,
Peres Carmem M.,
Roberto Mendonça José,
Curi Rui
Publication year - 2003
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.1048
Subject(s) - cholesterol , lipopolysaccharide , concanavalin a , incubation , chemistry , phorbol , endocrinology , in vitro , medicine , biochemistry , biology , protein kinase c , enzyme
The time‐course of incorporation of NBD‐cholesterol by macrophages (Ma) and lymphocytes (LY) obtained from untreated and thioglycollate‐injected (thio) rats was investigated. NBD‐cholesterol incorporation was also examined in Ma obtained from untreated rats and stimulated in vitro by lipopolysaccharide (LPS) and phorbol‐myristate acetate (PMA). The same measurement was performed in LY from untreated rats stimulated by addition of LPS and concanavalin A (Con A) into the culture medium. Thio‐treated Ma showed high fluorescence intensity after 1 h of incubation with NBD‐cholesterol. Ma submitted concomitant to LPS and NBD‐cholesterol showed low fluorescence intensity, as well as Ma stimulated with PMA. Ma from untreated and LPS pre‐treated rats showed a similar time‐course of incorporation. LY from thio‐treated rats showed lower incorporation of NBD‐cholesterol in comparison to LY from untreated rats. Incorporation was reduced when LPS was added concomitantly with NBD‐cholesterol. On the other hand, LY pre‐treated with LPS for 48 h showed a very high incorporation of NBD‐cholesterol. Con A treatment did not cause a significant effect on NBD‐cholesterol incorporation. The findings presented herein led us to conclude that the uptake of NBD‐cholesterol by Ma and LY is markedly affected by the activation state of the cells. Copyright © 2003 John Wiley & Sons, Ltd.