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Liquid chromatography on triacetylcellulose, 4. Determination of enantiomeric purity in spite of incomplete chromatographic separation
Author(s) -
Mannschreck Albrecht,
Eiglsperger Alfred,
Stühler Georgine
Publication year - 1982
Publication title -
chemische berichte
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 0009-2940
DOI - 10.1002/cber.19821150432
Subject(s) - chemistry , chromatography , enantiomer , optically active , chromatographic separation , analytical chemistry (journal) , stereochemistry , high performance liquid chromatography , organic chemistry
Abstract A novel procedure for the measurement of enantiomeric purity P is derived, whereby the mixture to be analyzed is subjected to liquid chromatography (LC) on an optically active sorbent. Extensive peak overlap does not prohibit the application of the method, whereas the complete absence of separation does. In addition, the mixture is subjected to LC on an achiral sorbent. The same photometer and polarimeter detections are used for both experiments. At the same time, an x/y recorder plots the rotation angle α versus the absorbance A , thus generating the slopes C m and C + in Fig. 1 which result in P = C m / C + . – Optically active samples of 1 , 2 , 3 , and 4 were analyzed for P. The findings agree satisfactorily with the results of other methods.