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Research Progress and Prospects for Fructosyltransferases
Author(s) -
Wang Yitian,
Shang Xiujie,
Cao Fan,
Yang Haiquan
Publication year - 2021
Publication title -
chembioeng reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.089
H-Index - 22
ISSN - 2196-9744
DOI - 10.1002/cben.202000011
Subject(s) - heterologous , mutagenesis , microorganism , heterologous expression , protein engineering , computational biology , biochemical engineering , protein expression , metabolic engineering , sucrose , microbiology and biotechnology , biology , chemistry , biochemistry , engineering , mutation , recombinant dna , genetics , bacteria , gene , enzyme
Fructosyltransferases can be used to produce fructooligosaccharides from sucrose and are obtained from different sources, mostly microorganisms and plants. Heterologous expression and several mutagenesis methods were applied to improve the production of fructosyltransferases. The structures and characteristics of fructosyltransferases were investigated. Immobilization and protein engineering can improve their characteristics. In this review, screening and mutagenesis of microorganisms, heterologous expression, purification, characterization, structural analysis, immobilization, and protein engineering of fructosyltransferases are discussed. Future prospects in this field are also considered.