Premium
Current Progresses in Phytase Research: Three‐Dimensional Structure and Protein Engineering
Author(s) -
Chen ChunChi,
Cheng KuoJoan,
Ko TzuPing,
Guo ReyTing
Publication year - 2015
Publication title -
chembioeng reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.089
H-Index - 22
ISSN - 2196-9744
DOI - 10.1002/cben.201400026
Subject(s) - phytase , thermostability , monogastric , protein engineering , enzyme , chemistry , biochemistry , microbiology and biotechnology , food science , biology , nutrient , plant nutrition , organic chemistry
Phytase is one of the most important feed additive enzymes for monogastric animal, because it hydrolyzes the indigestible phytate in the cereal‐based feedstock to release phosphate as an essential nutrient. To understand its molecular machinery, the three‐dimensional structures of various types of phytases and complexes have been studied extensively. For commercial applications, important properties such as higher catalytic efficiency and higher thermostability are desired. Since a phytase with both beneficial characteristics is hardly found in nature, various protein engineering strategies are popular in modifying the existing enzymes with enhanced performance. In this review, the up‐to‐date status of phytase structural and engineering studies is summarized. In addition, structural perspectives of some engineered phytases with improved properties are also provided. These results broaden the understanding of phytases and will be important for phytase applications in the future.