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New Insights into the Biological Activity of Lichens: Bioavailable Secondary Metabolites of Umbilicaria decussata as Potential Anticoagulants
Author(s) -
Vaez Mohsen,
Javad Davarpanah Seyed
Publication year - 2021
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202100080
Subject(s) - lichen , chemistry , bioavailability , prothrombin time , partial thromboplastin time , clotting time , coagulation , botany , pharmacology , biology , medicine
This study reports the in vitro anticoagulation activity of acetonic extract (AE) of 42 lichen species and the identification of potential bioavailable anticoagulant compounds from Umbilicaria decussata as a competent anticoagulant lichen species. Lichens’ AEs were evaluated for their anticoagulant activity by monitoring activated partial thromboplastin time (APTT) and prothrombin time (PT) assays. A strong, positive correlation was observed between total phenolics concentration (TPC) of species and blood coagulation parameters. U. decussata was the only species with the longest clotting time in both APTT and PT assays. The research was moved forward by performing in vivo assays using rats. The results corroborated the dose‐dependent impact of U. decussata ’s AE on rats’ clotting time. Major secondary metabolites of U. decussata and their plasma‐related bioavailability were also investigated using LC‐ESI‐MS/MS. Atranol, orsellinic acid, D‐mannitol, lecanoric acid, and evernic acid were detected as possible bioavailable anticoagulants of U. decussata . Our findings suggest that U. decussata might be a potential anticoagulant lichen species that can be used for the prevention or treatment of coagulation‐related issues such as cardiovascular diseases (CVDs).