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In Vivo and in Silico Trypanocidal Activity Evaluation of (−)‐Cubebin Encapsulated in PLGA Microspheres as Potential Treatment in Acute Phase
Author(s) -
Neres Nayara B. R.,
Montagnini Daniel,
Ferreira Daniele S.,
Parreira Renato L. T.,
Orenha Renato P.,
Lima Thais C.,
Molina Eduardo F.,
Cunha Wilson R.,
Silva Márcio L. A.,
Esperandim Viviane R.
Publication year - 2021
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202100052
Subject(s) - benznidazole , parasitemia , trypanosoma cruzi , chemistry , in vivo , trypanocidal agent , inoculation , microsphere , pharmacology , chagas disease , immunology , parasite hosting , biology , malaria , microbiology and biotechnology , chemical engineering , world wide web , computer science , plasmodium falciparum , engineering
In this study, the trypomastigotes of a Y strain of Trypanosoma cruzi were inoculated intraperitoneally into male BALB/c mice weighing approximately 25 g each, which were divided into groups for evaluation of the trypanocidal activity. For the treatment of experimental groups, encapsulated and unencapsulated (−)‐cubebin, Benznidazole, and two groups as negative controls were used. The encapsulated (−)‐cubebin showed a 68.1 % encapsulation efficiency. The parasitemia peak of substances remained around the 9 th day after the observed reduction in the number of circulating trypomastigotes. The encapsulated (−)‐cubebin and (−)‐cubebin unloaded showed a decrease of 61.3 % and 58.5 % in the number of parasites as compared to the negative control, respectively. Moreover, animals treated with encapsulated (−)‐cubebin had a higher survival time as compared to the other groups. In conclusion, the results obtained were more promising for encapsulated (−)‐cubebin as compared to unloaded particles.