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The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice
Author(s) -
Niu Lulu,
Wu Yaru,
Liu Huiping,
Wang Qing,
Li Mengyu,
Jia Qi
Publication year - 2021
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202001010
Subject(s) - chemistry , polysaccharide , in vivo , apoptosis , mushroom , galactose , fucose , edible mushroom , monosaccharide , biochemistry , annexin , xylose , in vitro , food science , biology , fermentation , microbiology and biotechnology
In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×10 6 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.