Premium
Anthocyanins from Aronia melanocarpa Induce Apoptosis in Caco‐2 Cells through Wnt/β‐Catenin Signaling Pathway
Author(s) -
Wei Jie,
Yu Wenchen,
Hao Ruobing,
Fan Jungang,
Gao Jun
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202000654
Subject(s) - wnt signaling pathway , chemistry , apoptosis , catenin , caco 2 , western blot , colorectal cancer , cell cycle checkpoint , signal transduction , cell growth , cell , cell cycle , in vitro , cancer research , biochemistry , cancer , biology , gene , genetics
Colorectal cancer (CRC) is one of the most common malignant tumors in the world. In this study, the Caco‐2 in vitro cell model was used to study the effect and mechanism of Aronia melanocarpa (Michx.) Elliott anthocyanins (AMA) on colon cancer. The experimental results showed that the binding energy of anthocyanins on β‐catenin was in the range of −5.92 to 4.95 kcal/mol, with good low energy parameters and binding positions. AMA can inhibit cell proliferation and cause cell cycle arrest. RT‐PCR and Western blot results showed that AMA can reduce cytoplasmic β‐catenin and inhibit the expression of related proteins in Wnt/β‐catenin signaling pathway. This study revealed the AMA inhibitory effect and mechanism of malignant biological behavior of Caco‐2 cells, in order to provide theoretical basis for the prevention and treatment of colon cancer by Aronia melanocarpa (Michx.) Elliott.